Topical mosquito control product

ABSTRACT

A topical mosquito control product includes active and inert ingredients. The active ingredients include natural materials and may include soybean oil, citronella oil, peppermint oil, thyme oil, cinnamon oil and cedar wood oil. The inert ingredients include natural materials and may include shea, anise oil, basil oil, bee&#39;s wax, isopropyl alcohol, avocado oil, sunflower oil, turmeric, salt, ethyl ester and water.

CROSS-REFERENCE TO RELATED APPLICATIONS

The instant application claims priority to U.S. Provisional ApplicationSer. No. 62/281,347 filed Jan. 21, 2016 and U.S. Provisional ApplicationSer. No. 62/416,457 filed Nov. 2, 2016 the entire contents of which areincorporated herein by reference.

FIELD OF THE INVENTION

The instant application is directed to a formulation for a topicalmosquito repellent.

BACKGROUND OF THE INVENTION

Mosquito bites often cause annoying reactions in humans and animals.Additionally, mosquito bites are a vector for many types of diseaseincluding: malaria, Dengue Fever, Chikungunya, West Nile and Zikaviruses as well as other forms of disease.

There is a need to prevent mosquitos from biting a host to lessen thediscomfort of a bite reaction and to prevent the potential spread of adisease.

Accordingly, a natural non-toxic ingredient based topical product thatprevents bites to humans and animals would be desirable.

SUMMARY OF THE INVENTION

In one aspect, there is disclosed a topical mosquito control productthat includes active and inert ingredients. The active ingredientsinclude soybean oil and citronella oil and the inert ingredients includeshea, anise oil, basil oil and isopropyl alcohol.

In another aspect, there is disclosed a topical mosquito control productthat includes active and inert ingredients. The active ingredientsinclude soybean oil and citronella oil and the inert ingredients includebee's wax, anise oil, basil oil and isopropyl alcohol.

In yet another aspect, there is disclosed a topical mosquito controlproduct that includes active and inert ingredients. The activeingredients include soybean oil, citronella oil, peppermint oil andthyme oil and the inert ingredients include bee's wax, avocado oil andisopropyl alcohol.

In yet another aspect, there is disclosed a topical mosquito controlproduct that includes active and inert ingredients. The activeingredients include soybean oil, cinnamon oil, cedar wood oil andcitronella oil and the inert ingredients include bee's wax, sunfloweroil, turmeric, anise oil, basil oil, salt, ethyl ester, water andisopropyl alcohol.

Also disclosed is a method of repelling insects which includes applyingthe lotion compositions to a body and exposing insects to the lotion.

Additional features and advantages of mosquito control productsdescribed herein will be set forth in the detailed description whichfollows, and in part will be readily apparent to those skilled in theart from that description or recognized by practicing the embodimentsdescribed herein, including the detailed description which follows, theclaims, as well as the appended drawings.

It is to be understood that both the foregoing general description andthe following detailed description describe various embodiments and areintended to provide an overview or framework for understanding thenature and character of the claimed subject matter. The accompanyingdrawings are included to provide a further understanding of the variousembodiments, and are incorporated into and constitute a part of thisspecification. The drawings illustrate the various embodiments describedherein and together with the description serve to explain the principlesand operations of the claimed subject matter.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a graphical representation of an experimental apparatus usedfor testing;

FIG. 2 is a graphical depiction of an experimental apparatus positionedover a blood source;

FIG. 3 is a graphical representation of an experimental apparatusincluding a circulating pump providing warm water to the testingapparatus.

DETAILED DESCRIPTION OF THE EMBODIMENTS

Mosquito bites may transmit diseases and cause reactions to people thatare bitten. Various repellant compositions have been utilized in priorart repellant compositions. One such repellant, DEET, is often used inmosquito repellant compositions. DEET has been questioned as to itstoxicity in higher amounts for use with humans and other subjects. Thereis therefore a need in the art for an improved and safe topical mosquitorepellant composition.

In one aspect, there is disclosed herein a formulation that may beutilized as a topical lotion or spray to repel mosquitos. Theformulation may include organic and chemical-free compositions that willprotect humans and other animals from blood-seeking insects such asmosquitos.

Example 1

In one aspect the composition may include shea, soybean oil, isopropylalcohol, anise oil, citronella oil, and basil oil. The shea may bepresent in an amount of from 50 to 70% by weight. The soybean oil may bepresent in an amount of from 10 to 30% by weight. The isopropyl alcoholmay be present from 5 to 15% by weight. The anise oil may be dispersedin a solvent such as isopropyl alcohol and may be present from 1.5 to2.5% or at about 2% by weight. The citronella oil and basil oil may alsobe present in an amount of from 1.5 to 2.5% or at about 2% by weight.The weight percentages are based on a total weight of the composition.

As described above, the composition may include active ingredients andinactive ingredients. The soybean oil and citronella oil may beclassified as active ingredients wherein the shea, isopropyl alcohol,anise oil, and basil oil may be considered inert or inactiveingredients.

Example 2

In an alternate composition, the shea may be replaced by beeswax. Asdescribed above, the shea had a 3 to 1 ratio with respect to the soyoil. However, the beeswax may have an alternate ratio with respect tothe soy oil. In one aspect, the beeswax may be present in a 1 to 8 ratiowith respect to the soy oil.

The composition may be formed by heating the soy oil to approximately140° F. Next, the shea or beeswax is added to the composition. Next, thecitronella oil, basil oil, and anise oils are added to the compositionand mixed thoroughly. The composition is then allowed to cool to roomtemperature forming a stiff mass. Next, the composition is blended witha lotion substrate to form a cream lotion or spray. Various ratios ofthe composition and substrate may be utilized, for example a ratio of 50to 4, 48 to 6, and 50 to 8 may be utilized with respect to thecomposition and the substrate cream component.

Example 3

In another aspect, the composition may include bee's wax, soybean oil,edible oil such as avocado oil or sunflower oil, isopropyl alcohol,peppermint oil, citronella oil, and thyme oil. The bee's wax may bepresent in an amount of from 10 to 20% by weight. The soybean oil may bepresent in an amount of from 40 to 60% by weight. The avocado oil may bepresent in an amount of from 20 to 35% by weight. The isopropyl alcoholmay be present from 5 to 15% by weight. The peppermint oil may bepresent at about 1% by weight. The citronella oil may also be present inan amount of from 1.5 to 2.5% or 2% by weight. The thyme oil may bepresent at about 0.5% by weight. Additionally, the composition mayinclude powdered ingredients such as vanillin present at about 0.4% byweight and Lecithin present at about 0.1% by weight. The weightpercentages are based on a total weight of the composition.

As described above, the composition may include active ingredients andinactive ingredients. The soybean oil, peppermint oil, thyme oil andcitronella oil may be classified as active ingredients wherein the bee'swax, avocado oil and isopropyl alcohol may be considered inert orinactive ingredients.

The composition of example 3 was made according to the followingprocedure. The soybean oil and sunflower oils were heated from roomtemperature to 145 to 160 degrees F. in a mixing vat under agitation.The vanillin, lecithin and bee's wax were added to the composition whenthe temperature approaches 100 F. The mixture is heated and agitateduntil the mixture clears. Once the mixture clears heat is removed fromthe composition. Isopropyl alcohol is added at the temperature of 110 F.The mixture immediately cools to 100 F. At this time the peppermint oil,citronella oil, and thyme oil are added to the composition. Thecomposition is agitated and sheared for 30 minutes. After which time thelotion may be packaged.

Example 4

In another aspect, the composition may include the following formula:

Percentage by weight Density Weight Ingredient Active Soybean Oil 27.50.915 25.1625 PLUS-MINUS 35% Citronella Oil 4 0.855 3.42 PLUS-MINUS 40%Cinnamon Oil 4 1.04 4.16 PLUS-MINUS 40% Cedar Wood Oil 4 0.952 3.808PLUS-MINUS 40% Ingredient Inert Sunflower Oil 24 0.924 22.176 PLUS-MINUS35% Turmeric 4 0.92 3.68 PLUS-MINUS 40% Anise 3 0.98 2.94 PLUS-MINUS 20%Basil Oil 4 0.895 3.58 PLUS-MINUS 25% Beeswax 11 0.96 10.56 PLUS-MINUS30% Salt 1 2.165 2.165 PLUS-MINUS 20% Ethyl Ester 3.3 1.04 3.432PLUS-MINUS 15% Isopropyl Alcohol 3 0.785 2.355 PLUS-MINUS 20% Water 2 12 PLUS-MINUS 35% Vanillin 5 1.06 5.3 PLUS-MINUS 40% Lecithin 0.2 1.030.206 PLUS-MINUS 25%

The composition may include bee's wax, soybean oil, edible oil such assunflower oil, isopropyl alcohol, cinnamon oil, cedar wood oil,citronella oil, turmeric, anise, basil oil, salt, ethyl ester, andwater. The bee's wax may be present in an amount of from 10 to 20% byweight. The soybean oil may be present in an amount of from 10 to 60% byweight. The sunflower oil may be present in an amount of from 20 to 35%by weight. The isopropyl alcohol may be present from 2 to 10% by weight.The cinnamon oil may be present at about 2 to 10% by weight. Thecitronella oil may also be present in an amount of from 2% to 10% byweight. The cedar wood oil may be present in an amount of from 2% to 10%by weight. The turmeric may be present in an amount of from 2% to 10% byweight. The anise may be present in an amount of from 2% to 10% byweight. The basil oil may be present in an amount of from 2% to 10% byweight. Salt may be present in an amount of from 1% to 3% by weight. Theethyl ester may be present in an amount of from 2% to 10% by weight.Water may be present in an amount of from 1 to 4% by weight.Additionally, the composition may include powdered ingredients such asvanillin present at about 5% by weight and Lecithin present at about0.2% by weight. The weight percentages are based on a total weight ofthe composition.

As described above, the composition may include active ingredients andinactive ingredients. The soybean oil, cinnamon oil, cedar wood oil andcitronella oil may be classified as active ingredients wherein the bee'swax, sunflower oil, turmeric, anise oil, basil oil, salt, ethyl ester,water, vanillin, lecithin and isopropyl alcohol may be considered inertor inactive ingredients.

The composition of example 4 was made according to the followingprocedure. The soybean oil and sunflower oils were heated from roomtemperature to 145 to 160 degrees F. in a mixing vat under agitation.The vanillin, lecithin and bee's wax were added to the composition whenthe temperature approaches 100 F. The mixture is heated and agitateduntil the mixture clears. Once the mixture clears heat is removed fromthe composition. Water is then added to the composition under agitationand the composition is cooled to 110 F. Isopropyl alcohol is added atthe temperature of 110 F. The mixture immediately cools to 100 F. Atthis time the cinnamon oil, cedar wood oil, turmeric, anise oil, basiloil, ethyl ester and an emulsion of salt water and citronella oil areadded to the composition. The emulsion includes a 3.5% salt watermixture mixed at a ratio of 4 to 1 with the citronella oil. Thecomposition is agitated and sheared for 30 minutes. After which time thelotion may be packaged.

The deterrent effect of the above-described compositions of examples 1-3 was tested in a controlled environment. In vitro laboratory testswere conducted and measured and compared to DEET and nontreated controlgroups for yellow fever mosquitos, Aedes aegypti. Bioassays wereconducted in nine six-chambered Plexiglas K & D modules stocked withfemale Aedes aegypti mosquitos as shown in FIGS. 1-3. Five femalemosquitos were aspirated into each of the six chambers of the ninemodules. Repellant treatments were applied at 26.7 microliters with apipette and spread with a small brush over 12 square centimeter sectionsof organdy cloth attached to a flat plastic template containingrectangular openings. The treated cloth covered template was fittedbetween the K & D module and a lower Plexiglas unit. The lower Plexiglasunit contained shallow surface wells filled with a blood substitute(CDTA and ATP) and covered with a moistened collagen membrane. The bloodsubstitute was heated to 38° C. using water pumped through hose linesattached to the lower units and a water bath supplied with a temperaturecontrolled inversion circulator. Mosquitos were exposed to the treatedsurfaces by opening sliding doors beneath the modules for 90-secondbiting counts at five post-treatment time intervals from 1 to 6 hours.Fresh, untreated mosquitos are used for each time interval.

The bite counts were tabulated and utilized for statistical analysis.The percent repellency of the formulations as described above was moreeffective than DEET over the five time periods. At the initial starttime of zero the repellency of the composition as described herein was100% while DEET was 91.9%. The average for all five time periodsdemonstrates the repellency of 94.54% for the compositions as describedherein compared to 86.52% for DEET. At the 6 hour time period thecompositions as described herein included a repellency of 96.8%effective compared to DEET which was 87.1% effective.

The testing protocol including the in vitro environment provides arigorous testing of the repellency of the composition as the mosquitosare confined with a blood meal.

An unpaired t-test was conducted on the biting counts between lotion asdescribed herein and the control group where there was no treatmentapplied between the mosquito and the blood well. The results of thistest showed highly significant results between the mean biting counts of0.33 for the compositions as described herein compared to 3.44 for thecontrol group. The statistical factors include t=5.8704 where df=16 anda standard error of difference is equal to 0.530. The two-tailed p valueis less than 0.0001. The reduction in biting pressure when using thecompositions compared to the control group is significant at the 99.99level of significance.

The experimental results demonstrate a very high effectiveness ofrepellency utilizing a safe and effective organic product that is bothDEET and chemical free. The testing has demonstrated that the product iseffective in repelling mosquitos and in particular the yellow fevermosquito which may potentially carry West Nile virus.

Additional testing was performed with the lotion formula of example 4including: bee's wax, soybean oil, edible oil such as sunflower oil,isopropyl alcohol, cinnamon oil, cedar wood oil, citronella oil,turmeric, anise, basil oil, salt, ethyl ester, and water with thefollowing protocols. Aedes aegypti mosquitoes were obtained frominsecticide-susceptible reference strains held at the London School ofHygiene & Tropical Medicine, UK. All mosquitoes are reared and housedunder optimal environmental conditions of 25° C.±2° C. and 80% RelativeHumidity with a 12:12 hour photoperiod. Testing was carried out in atesting room maintained at 25±2K and 80% RH with a 12:12 hourphotoperiod. For each product, the same person carried out thearm-in-cage testing.

For each product test, three batches of 50 female mosquitoes were placedin cages 30×30×30cm inside the testing room. Before the start of eachtest a bare arm with gloved hand was inserted into the cage for 30seconds to assess the biting readiness of the mosquitoes. Only cageswith at least ten mosquitoes landing within 30 seconds were used insubsequent testing. The number of mosquitoes probing the untreated armwas counted at the end of the 30 second test. Following the combinedfitness/control test, the product was applied onto the right arm at theWHO standard rate of 1 ml per 600 cm2 (1.6711 per crn2) for the MosquitoInsect Repellent and DEET, and at a rate of 2 mg per cm' for theSunscreen Repellent. The arm was then inserted into the cage for 30seconds and the number of mosquitoes probing on the arm counted andrecorded. The procedure was repeated with a total of three cages. Thiswas repeated for each of the three cages and at hourly intervals until 8hours post-application or until protection drops below 50%. Each productwas tested on a separate test day.

Protective efficacy (PE) was calculated using the following formula:PE=(Mosquitoes probing on control arm−Mosquitoes probing on treatedarm)/Mosquitoes probing on control arm×100. The average probing on thecontrol arm during testing was 16, 17 and 21 mosquitoes at the end ofthe 30 second exposure time, in cages used for testing the MosquitoInsect Repellent Lotion and 8% DEET respectively. The Mosquito InsectRepellent Lotion 100% protection at 0 hours, whereas the 8% DEETprovided 100% protection for 1 hour after application (Table 1). TheMosquito Insect Repellent Lotion provided over 50% protection for, 3hours, whereas 8% DEET provided over 50% protection for 6 hours.

TABLE 1 Total Protective Efficacy of the Mosquito Insect RepellentLotion against Aedes aegypti mosquitos No. No. probing probing on bareon Hours after Time control treated Total Application (hh:mm) Replicatearm arm % PE % PE 0 10.44 1 13 0 100.00 100.00 2 15 0 100.00 3 12 0100.00 1 11:43 1 10 1 90.00 90.91 2 16 1 93.75 3 18 2 88.89 2 12:48 1 120 100.00 86.96 2 15 2 86.67 3 19 4 78.95 3 13:43 1 14 5 64.29 62.22 2 125 58.33 3 19 7 63.16 4 14:45 1 16 6 62.50 47.83 2 18 10 44.44 3 12 833.33 5 15:45 1 17 13 23.53 31.03 2 20 14 30.00 3 21 13 38.10

COMPARATIVE TABLE 2 Total Protective Efficacy of 8% DEET against Aedesaegypti mosquitos No. No. probing probing on bare on Hours after Timecontrol treated Total Application (hh:mm) Replicate arm arm % PE % PE 009:40 1 16 0 100.00 100.00 09:42 2 28 0 100.00 09:44 3 30 0 100.00 110:40 1 15 0 100.00 100.00 10:42 2 23 0 100.00 10:43 3 35 0 100.00 211.42 1 12 1 91.67 95.00 11:44 2 22 2 90.91 11:46 3 26 0 100.00 3 12:401 ND ND — 95.35 12:42 2 20 2 90.00 12:46 3 23 0 100.00 4 01:40 1 24 291.67 95.15 01:42 2 17 1 94.12 01:44 3 21 0 100.00 5 02:40 1 29 2 93.1090.00 02:42 2 18 2 88.89 02:44 3 23 3 86.96 6 03:40 1 17 10 41.18 54.9003:42 2 14 6 57.14 03:44 3 20 7 65.00 7 04:40 1 11 7 36.36 46.34 04:42 212 6 50.00 04:44 3 18 9 50 00

Additional Testing

Additional field testing of the lotion formulations was performed.

Sarasota, Fla.

Two years of testing in a swamp-type environment in central Sarasota,Fla. from 2014 through 2016 yield 100% effectiveness in a naturalenvironmental setting with high mosquito infestation. After applicationof the lotion of examples 1-4 as presented in the embodiments disclosedabove, it was observed that the repellency is 100% and with noapplication of the product the number of bites normally exceeds 10 in a5 minute exposure.

Company personnel camping in Myakka State Park in central Florida havetested the lotions over numerous overnight camping trips over the past 2years (2014 to 2016). The Myakka River flows through 57 square miles ofwetlands and prairies and may rival the Cagayan Region in northern Luzonin the Philippines for mosquito population densities. During these fieldtests the personnel report 100% success in repellency.

Tauo, Cagayan Valley, Philippines

During January of 2016 concrete workers on the edge of flooded ricefields in Tauo were given the lotion of the embodiments of examples 1-4described above. During an 8 hour day these workers reported no bites,or 100% repellency. Other tile and wood workers at the same locationworking on the project were given no repellent and experienced scores ofbites. For the following days of the construction project all workerswere given the lotion and no workers reported bites.

We claim:
 1. A topical mosquito repellent composition comprising: alotion including active and inert ingredients, wherein the activeingredients include soybean oil and citronella oil and the inertingredients include shea, anise oil, basil oil and isopropyl alcohol. 2.The topical mosquito repellent composition of claim 1 wherein shea ispresent in an amount of from 50 to 70% by weight, soy bean oil ispresent in an amount of from 10 to 30% by weight, isopropyl alcohol ispresent in an amount of from 5 to 15% by weight, anise oil is present inan amount of from 1.5 to 2.5% by weight, anise oil is present in anamount of from 1.5 to 2.5% by weight, and citronella oil is present inan amount of from 1.5 to 2.5% by weight.
 3. A topical mosquito repellentcomposition comprising: a lotion including active and inert ingredients,wherein the active ingredients include soybean oil and citronella oiland the inert ingredients include bee's wax, anise oil, basil oil andisopropyl alcohol.
 4. The topical mosquito repellent composition ofclaim 3 wherein bee's wax is present in an amount of from 10 to 20% byweight, soy bean oil is present in an amount of from 40 to 60% byweight, isopropyl alcohol is present in an amount of from 5 to 15% byweight and citronella oil is present in an amount of from 1.5 to 2.5% byweight.
 5. A topical mosquito repellent composition comprising: a lotionincluding active and inert ingredients, wherein the active ingredientsinclude soybean oil, citronella oil, peppermint oil and thyme oil andthe inert ingredients include bee's wax, avocado oil and isopropylalcohol.
 6. The topical mosquito repellent composition of claim 5wherein bee's wax is present in an amount of from 10 to 20% by weight,soy bean oil is present in an amount of from 40 to 60% by weight,avocado oil is present in an amount of from 20 to 35% by weight,isopropyl alcohol is present in an amount of from 5 to 15% by weight,citronella oil is present in an amount of from 1.5 to 2.5% by weight,peppermint oil is present in an amount of about 1% by weight, and thymeoil is present in an amount of about 0.5% by weight.
 7. The topicalmosquito repellent composition of claim 5 wherein the inert ingredientsfurther includes powdered ingredients including vanillin and lecithin.8. The topical mosquito repellent composition of claim 7 wherein thevanillin is present in an amount of about 0.4% by weight and lecithin ispresent in an amount of about 0.1% by weight.
 9. A topical mosquitorepellent composition comprising: a lotion including active and inertingredients, wherein the active ingredients include soybean oil,cinnamon oil, cedar wood oil and citronella oil and the inertingredients include bee's wax, sunflower oil, turmeric, anise oil, basiloil, salt, ethyl ester, water and isopropyl alcohol.
 10. The topicalmosquito repellent composition of claim 9 wherein the bee's wax ispresent in an amount of from 10 to 20% by weight, the soybean oil ispresent in an amount of from 10 to 60% by weight, the sunflower oil ispresent in an amount of from 20 to 35% by weight, the isopropyl alcoholis present from 2 to 10% by weight, the cinnamon oil is present in anamount of from 2 to 10% by weight, the citronella oil is present in anamount of from 2% to 10% by weight, the cedar wood oil is present in anamount of from 2% to 10% by weight, the turmeric is present in an amountof from 2% to 10% by weight, the anise oil is present in an amount offrom 2% to 10% by weight, the basil oil is present in an amount of from2% to 10% by weight, salt is present in an amount of from 1% to 3% byweight, ethyl ester is present in an amount of from 2% to 10% by weight,water is present in an amount of from 1 to 4% by weight.
 11. The topicalmosquito repellent composition of claim 9 wherein the inert ingredientsfurther includes powdered ingredients including vanillin and lecithin.12. The topical mosquito repellent composition of claim 9 wherein thevanillin is present in an amount of about 5% by weight and lecithin ispresent in an amount of about 0.2% by weight.
 13. A method of repellinginsects comprising: applying a lotion composition to a body and exposinginsects to the lotion, the lotion including active and inertingredients, wherein the active ingredients include soybean oil andcitronella oil and the inert ingredients include shea, anise oil, basiloil and isopropyl alcohol.
 14. The method of claim 13 wherein shea ispresent in an amount of from 50 to 70% by weight, soy bean oil ispresent in an amount of from 10 to 30% by weight, isopropyl alcohol ispresent in an amount of from 5 to 15% by weight, anise oil is present inan amount of from 1.5 to 2.5% by weight, anise oil is present in anamount of from 1.5 to 2.5% by weight, and citronella oil is present inan amount of from 1.5 to 2.5% by weight.
 15. A method of repellinginsects comprising: applying a lotion composition to a body and exposinginsects to the lotion, the lotion including active and inert ingredientswherein the active ingredients include soybean oil and citronella oiland the inert ingredients include bee's wax, anise oil, basil oil andisopropyl alcohol.
 16. The method of claim 15 wherein bee's wax ispresent in an amount of from 10 to 20% by weight, soy bean oil ispresent in an amount of from 40 to 60% by weight, isopropyl alcohol ispresent in an amount of from 5 to 15% by weight and citronella oil ispresent in an amount of from 1.5 to 2.5% by weight.
 17. A method ofrepelling insects comprising: applying a lotion composition to a bodyand exposing insects to the lotion, the lotion including active andinert ingredients, wherein the active ingredients include soybean oil,citronella oil, peppermint oil and thyme oil and the inert ingredientsinclude bee's wax, avocado oil and isopropyl alcohol.
 18. The method ofclaim 17 wherein bee's wax is present in an amount of from 10 to 20% byweight, soy bean oil is present in an amount of from 40 to 60% byweight, avocado oil is present in an amount of from 20 to 35% by weight,isopropyl alcohol is present in an amount of from 5 to 15% by weight,citronella oil is present in an amount of from 1.5 to 2.5% by weight,peppermint oil is present in an amount of about 1% by weight, and thymeoil is present in an amount of about 0.5% by weight.
 19. The method ofclaim 17 further including vanillin present in an amount of about 0.4%by weight and lecithin present in an amount of about 0.1% by weight. 20.A method of repelling insects comprising: applying a lotion compositionto a body and exposing insects to the lotion, the lotion includingactive and inert ingredients, wherein the active ingredients includesoybean oil, cinnamon oil, cedar wood oil and citronella oil and theinert ingredients include bee's wax, sunflower oil, turmeric, anise oil,basil oil, salt, ethyl ester, water and isopropyl alcohol.
 21. Themethod of claim 20 wherein the bee's wax is present in an amount of from10 to 20% by weight, the soybean oil is present in an amount of from 10to 60% by weight, the sunflower oil is present in an amount of from 20to 35% by weight, the isopropyl alcohol is present from 2 to 10% byweight, the cinnamon oil is present in an amount of from 2 to 10% byweight, the citronella oil is present in an amount of from 2% to 10% byweight, the cedar wood oil is present in an amount of from 2% to 10% byweight, the turmeric is present in an amount of from 2% to 10% byweight, the anise oil is present in an amount of from 2% to 10% byweight, the basil oil is present in an amount of from 2% to 10% byweight, salt is present in an amount of from 1% to 3% by weight, ethylester is present in an amount of from 2% to 10% by weight, water ispresent in an amount of from 1 to 4% by weight.
 22. The method of claim20 further including vanillin present in an amount of about 5% by weightand lecithin present in an amount of about 0.2% by weight.